2 calculation questions
In the protocol that I am using it says, make a working concentration of 25 mM and while adding it to my solution of 10 ml, it says add 50 mg.
Can someone please explain how can I do this? i.e. after dissolving the powder to achieve a concentration of 25 mM, how can I use it at 50 mg? The molar mass is 185 g/mol
My antibody data sheet tells me that the concentration is 1.05 µg/µl. And I want to use the antibody at 1:750 concentration. Can you please tell me how can I convert the ratio to µg/ml or vice versa.