Western Blot - wrong size bands
I'm running a Western Blot with rat Hippocampus samples and a Sigma TLR 7 antibody (http://www.sigmaaldrich.com/catalog/product/sigma/wh0051284m4?lang=de®ion=DE). The problem is I didn't get the band at 121 Kda but at 50 Kda (as shown on the Sigma Website).
What could be wrong? Could the ladder be wrong or not representing the correct size or something wrong with the samples?