4 Comments

Go to the profile of mdfenko
mdfenko 7 months ago

you can tag the nucleotides with fluorescent or radioactive labels

Go to the profile of thelongwindingcode
thelongwindingcode 7 months ago

Thank you for your response!
This is what I was thinking, do you know if cy3 and cy5 are FRET pairs? The literature seems to suggest they are, but people are still using them to quantify numbers of molecules in the same sample.

Go to the profile of mdfenko
mdfenko 7 months ago

that's the pair i used when 2-color hybridizing microarrays.

Go to the profile of thelongwindingcode
thelongwindingcode 7 months ago

Excellent! Thank you!