4 Comments

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mdfenko 10 months ago

you can tag the nucleotides with fluorescent or radioactive labels

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thelongwindingcode 10 months ago

Thank you for your response!
This is what I was thinking, do you know if cy3 and cy5 are FRET pairs? The literature seems to suggest they are, but people are still using them to quantify numbers of molecules in the same sample.

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mdfenko 10 months ago

that's the pair i used when 2-color hybridizing microarrays.

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thelongwindingcode 10 months ago

Excellent! Thank you!