Question about DNA extraction and PCR
Hi I am a new one in DNA extraction and PCR.
I extracted DNA from mouse tail which should contain one control Genome and one (or two) of two known target Genomes.
After PCR, the control genome always showed in Electrophoresis which indicate the exsitance of the chromosome. However, none of the target genomes showed in the electrophoresis result.
I also did sample of the two target genomes with the same primers I used and both showed in electrophoresis result. So it should not be primier issue either.
Can anyone help me check anyother posibility of not-showup of the target genomes?