Reconstitution and dilution of cytokines
Im am making growth media for single cell cultures.
I need to ad cytokines in very small concentrations and volumes.
I need to reconstitute 2 ug of a cytokine to 0.1-1.0 mg/mL.
Furthermore I need to dilute 0.1-1.0 mg/mL to a stock concentration of 10000 ng/mL
Can anybody help me on how to do this?