Buffer with multiple ingredients
I got a modified CTAB protocol and I need to prepare the following Nuclei extraction buffer:
10 mM TRIS-HC1 pH 9.5,
10 mM EDTA pH 8.0,
100 mM KC1,
500 mM sucrose,
4 mM spermidine,
1 mM spermine,
And the protocol says, add 10 ml of ice-cold Nuclei extraction buffer.
I am unable to figure out how to prepare the buffer solution. I need to prepare 500ml.
Thanks in advance and look forward to your feedback please.