I obviously have problem with molarity. I need help with understanding this, I need 1:1 molar ratio and 1:5 molar ratio of protein and dye.
I have 9.2mM dye and 40uM protein sample. Once someone told me to get 1:1 ratio I need to use 2ul of this dye to this sample of protein and (of course) if I want 1:5 ratio then I would use 10ul of this dye. I do not understand the calculation behind this, seems wrong to me. Can someone please explain this to me? If it is possible please use example.