Protein concentration assays
I have question about interference during protein concentration assays. I have my protein purified but my sample also contains 5 mM ATP during the experiment. I am quiet sure that ATP interferes with Nanodrop when I want to check concentration of my protein in the end. I expect low concentration and I get extremely high values (even if I blank the Nanodrop with only buffer containing ATP). My question is how can I find out concentration of my protein? Does ATP interferes with Bradford? I searched and I found mixed information.