DEAE cellulose is positively charged at neutral pH and therefore binds negative charged proteins.
where is Ion exchange here taking place. (can anyone pls direct me to a picture where resin functional group interacts with a protein)
If a Ca+ ions replaces Na+ mobile ion that is bound to the moiety of the resin it makes sense there is an exchange of strong affinity vs weak affinity ion.
I don't understand when this come to proteins especially with Q column
Quaternary ammonium binds to negative charge protein, can maintain their charge density over a pH range of 0–14. So where does ion exchange takes place?