Bizarre CRISPR/Cas9 expression of Cas9 RNA or sgRNA not both
Hi a CRISPR/Cas9 question if anyone can help (please see attached photo for better clarity):
We have 36 transformed wheat lines with CRISPR/Cas9 and tested in those lines which had been successfully transformed with the Cas9 RNA and the sgRNA (the two things needed) but (as in the picture) we had several plants that expressed Cas9 but not guide RNA and vice versa, even though they were transformed in equal proportions.
Is there a reason why some plants have Cas9 mRNA (including those that were the highest expressers- starred) but not guide RNAs (e.g. no.3), or why did some plants have almost all the guide RNA's expressed but no mRNA (e.g.14, 4)? Or is this chance?
Thank you so so much for any help!