bacillus transformation

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Dec 30, 2017
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Hi all,

I have been trying to transform my vector construct to bacillus for the past 2 months,i tried electroporation and some chemical method. I could not get anything on the plat. If anyone knows other good procedure to do this, please help me out.


Thanks!

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OldCloner 11 months ago

Hi Marissa,

I’ve never done Bacillus transformation, but I found some articles that might be helpful, including the one at the link below. What species (subtilis?) and strain are you using?

https://www.frontiersin.org/articles/10.3389/fmicb.2015.01431/full

If this one isn’t appropriate, try searching your specific species name, strain and “transformation protocol” and see if you can find any workable protocols.

Hope this helps!