generating abiraterone resistant prostate cancer cell lines

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Nov 23, 2017
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I have read several papers that have generated abiraterone resistant C4-2 cells, but do not explain how they generate these cell lines. I would like to generate abiraterone resistant C4-2 cells and I am wondering what would be the best approach for 1.) concentration(s) of Abiraterone to use 2.) how ofter fresh Abiraterone should be applied

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