Reconstitution and dilution of cytokines

Go to the profile of sciencetool
Aug 14, 2017
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Hi

Im am making growth media for single cell cultures.
I need to ad cytokines in very small concentrations and volumes.

I need to reconstitute 2 ug of a cytokine to 0.1-1.0 mg/mL.
Furthermore I need to dilute 0.1-1.0 mg/mL to a stock concentration of 10000 ng/mL

Can anybody help me on how to do this?


Kind regards,

3 Comments

Go to the profile of mdfenko
mdfenko 8 months ago

the magic formula for dilutions is:

c1v1=c2v2

2ug is .002mg, to prepare 1mg/ml...

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sciencetool 8 months ago

So to get a concentration of 0.1 mg/mL I would have to add 20 uL of water right?

To go from a concentration of 0.1 mg/mL to 10000 ng/mL:

0.1 mg = 0.1x10^-6

I would like a final volume of 50 uL

Using the formula:

0.1x10^6 ng/mL * V1 = 10000 ng/mL * 0.05 mL

V1 = 0.005 mL

Is that right?

Go to the profile of mdfenko
mdfenko 8 months ago

yes
10000ng/ml is 10ug/ml; 0.1mg/ml=100ug/ml; your stock is 10x; you got the right answer (5ul to make 50ul) but watch out for your units, you got some of them a little screwy.