Lysis buffer preparation
I've been away from molecular biology for a while and I just want some help with calculations. I need to prepare a lysis buffer for single cells. The guidelines I have are as follows: The requiredlysisbuffer canbe made by combining 0.2% (vol/vol) Triton X-100 and 2 U/µl RNase inhibitor.
I need to freeze my cells in 2.3µl lysisbuffer. The RNase inhibitor I will order is 100 µL at 20 U/µL.
Please walk me through how to prepare the lysis buffer. Thanks.