statistical test for ELISA results

Go to the profile of laleh
Apr 18, 2012
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4

Hi
I investigated effects of a certain chemical agent on recombinant protein productivity in a CHO cells stable cell line expression erythropoietin. After treatment, I performed ELISA test to investigate positive or negative effects of the agent. I had three group of control cells and three of test cells and ELISA tests were carry out in triplicate for each sample. I am not so familiar with statistics and I do not know what kind of test should I run for my experiment.


here there are my results:

control1: 463 ng/ml
control 2 466 ng/ml
control 3 468 ng/ml


test 1: 600 ng/ml
test 2: 640 ng/ml
test 3: 580 ng/ml

I appreciate any advise.

Bests,

4 Comments

Go to the profile of aarfy
aarfy 7 months ago

Probably the most basic test you could run is a T-test which will output a probability value of how likely your results are due to random events versus an actual event due to your treatment.

I quickly ran your results, and using a 2-tailed, T-test, you have a p-value = 0.015. So there's a 1.5% chance that your cells randomly produced more protein. So depending on where you place your p-value cut-off (most common is 0.05) will determine whether your treatment had significant value. I would say "yes".

Go to the profile of laleh
laleh 7 months ago

thank you very much for your answer, I have one more question: can we use parametric tests like t-test for small sample size like this situation?

Go to the profile of randolphbenjamin
randolphbenjamin 7 months ago

I don't think you can but I would like an answer to this myself. I'm not sure the t-test will work on such small sample sizes.

Go to the profile of arynews
arynews 7 months ago

thank you very much for your answer, I have one more question: can we use parametric tests like t-test for small sample size like this situation?