One of the most widespread protein laboratory accessory is the MWCO (molecular weight cut-off) centrifugal filters which is commonly used for concentrating protein and DNA too. They are available commercially with different cut-offs like 3kDa, 30kDa, 50kDa, 100kDa and so on. These little devices are expensive and hence demands proper usage and care to utilise its benefit to full extent. Here, I am going to discuss some tips and trick pertaining to its storage, use, and finally some troubleshooting inputs gathered from my experiences with centrifugal filters.

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