baculovirus lost titer

Go to the profile of volodya_tx
Oct 06, 2017

Dear Colleagues,

My baculoviral P3 stock stopped working for protein expression (storage time ~5 months at 4 C).
I tried to re-amplify from P2->P3 virus and P3->P4 viruses (also stored for 5 months at 4 C), my cells got infected and then I harvested supernatant 120 h post-infection (cell viability was ~30%), but when these viruses were used for expression they did not work again.
I feel I should start all over again from bacmid transfection?


Go to the profile of anorak
anorak 8 months ago

Hey, It might be a long shot, but I have similar problem at the moment and I don't really know what to do? Have you found some emergency solution or you just started over?