doing a polyA capture twice - does it work?
Has anyone ever done a poly-A capture twice on mRNA material? Has your recovery been good? I have mRNA enriched material from another source and I need to use it as input into another protocol but the input requires it to be in the protocol specified buffer solution. I would like to recapture the material so that it is eluted in the protocol specified buffer solution (which contains enzymes and some cocktail of reagents, not just a simple buffer). In essence, I will be doing the polyA twice. Has anyone done polyA capture twice and has it worked for you?