qPCR reaction with probe self-dimer

Go to the profile of namtran93
May 10, 2017
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1

Hello everyone,

I am trying to optimize a qPCR reaction. Unluckily, I realize that my probe form a self-dimer structure which impair the signal of amplification. How can I improve my qPCR reaction? I hope that someone can help me.

1 Comments

Go to the profile of drshabba
drshabba 9 months ago

How did you discover the self-dimer, are you sure that is impairing the reaction?

What type of fluorophore and quencher are you using?

Redesign your probe if possible - if you can't change the sequence then try ordering from a different company