Qiagen gDNA Wipeout Buffer Composition

Go to the profile of ck24
Aug 01, 2017
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Help! Does anyone here know any of the components of gDNA Wipeout Buffer? Qiagen's QuantiTect Reverse Transcription Kit requires that the reverse transcription be done in the presence of the gDNA Wipeout Buffer, but I can't figure out how they are capable of degrading gDNA without a DNase and just with buffers. There must be something in the gDNA Wipeout Buffer that isn't in the reverse transcription buffer, but is necessary for proper function of the reverse transcriptase.. Any ideas?

2 Comments

Go to the profile of drshabba
drshabba 9 months ago

Looking at their protocol, it must have DNA double-strand-specific endonuclease in the wipeout buffer (dsDNase). This specifically targets the double stranded DNA in the reaction whilst leaving the cDNA alone

QIAGEN are very good at hiding what the composition of their buffers are - it was hard enough when I worked for them...

I hope this helps

Go to the profile of relaxin
relaxin 9 months ago

Haha, that is the secret. dsDNase is available from ThermoFisher, it comes with buffer.

I am retired, never work with ThermoFisher.

https://www.thermofisher.com/order/catalog/product/EN0771