Does high ROX levels invalidates a qPCR assay ?
We are having problems with ROX levels (higher than SYBR Green when visualized the Multicomponent plot) when using some qPCR chemistries. We know there are different formulations not interexchangeables for different qPCR devices. My question is:
How these anormal high levels of ROX do affect the Ct obtained ? Do they simply reduce the sensibility of the assay ? Do they fully invalidate the results ?
Thanks in advance,