PCR for Toll-like receptors 4

Go to the profile of zita
Jun 24, 2017
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Hello friends,
I am looking for some protocol which will help me to increase intensity of PCR for TLR-4. Can someone help me with this issue please? It's quite urgent

2 Comments

Go to the profile of Roberto Rosati
Roberto Rosati 9 months ago

Hi,
I think you'll get replies if you post more information, but how can we help if we don't know anything about your assay?

Go to the profile of zita
zita 9 months ago

I am sorry, I was not very specific. 
So the problem is that curve for TLR4 is coming up in 35th cycle which is late. Probably the quantity of RNA is low or there could be some contamination, so I am looking for some protocol or tip, which will help me to make whole reaction more sensitive. 
We can use more concentration of RNA but we would like to find another possibilities.