artifacts in sequencing gels
I am using sequencing gels to separate radiolabeled (32P dATP) DNA fragments that are generated during DNA repair. I often encounter smear-like artifacts in empty lanes (that are not loaded with any samples) close to loaded lanes. This prevents me from being able to interpret the data. Does anyone know the cause of this kind of artifact and how It can be prevented?
I run 4 mm thick 7% polyacrylamade gels using the S2 sequencing gel electrophoresis apparatus and shark tooth combs.