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Cell Culture

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Selective media for potential polystyrene plastic-degrading bacteria

Greetings! I’m a student and for our research we’re attempting to isolate potential plastic-degrading bacteria from sediment samples on the trash-filled coast of an island. We’re having trouble in what our media should be. We know it should be selective since it will eliminate those bacteria that don’t use plastic as a carbon source but we don’t know a specific formula or kind of media to use. Any advice? Thank you in advance!
Go to the profile of JM Quismundo
Jan 22, 2019

Protein Methods

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Spinning the centrifugal filters: Tips and Tricks

One of the most widespread protein laboratory accessory is the MWCO (molecular weight cut-off) centrifugal filters which is commonly used for concentrating protein and DNA too. They are available commercially with different cut-offs like 3kDa, 30kDa, 50kDa, 100kDa and so on. These little devices are expensive and hence demands proper usage and care to utilise its benefit to full extent. Here, I am going to discuss some tips and trick pertaining to its storage, use, and finally some troubleshooting inputs gathered from my experiences with centrifugal filters.
Go to the profile of Haaris Ahsan Safdari
Mar 08, 2019